Anti-Collagen type II [CIIC1 ]

Absolute Antibody

Anti-Collagen type II [CIIC1 ]

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This anti-collagen type II antibody CIIC1 is an arthritogenic antibody, which induces arthritis in mice (Holmdahl et al., 1986; PMID: 2421741 and Nandakumar et al., 2003; PMID: 12884302). The antibody specificity towards collagen type II was assayed in an ELISA. The antibody was selected for in vivo injection experiments in naive DBA/1 male mice, at no time was any arthritis detected macroscopically after treatment with 0.5, 2 or 5 mg of antibody per animal, however development of synovitis was noticed (Holmdahl et al., 1986; PMID: 2421741). The structure of the Fab fragment was determined by X-ray crystallography. The specificity of the full length CIIC1 antibody and the FV fragment was confirmed by ELISA analysis. Further investigation by using both the full length antibody and a single chain Fv fragment in ELISA experiments revealed that the antibody bound also to other IgG1, IgG2a and IgG2b anti-collagen antibodies (Uysal et al., 2008; PMID: 18241923). The antibody detected collagene type II by western blot analysis (Burkhardt et al., 2005; PMID: 15832289). In vitro studies demonstrated that the antibody together with M2139 antibody suppressed the self-assembly of CII into fibrils (Croxford et al., 2010; PMID: 20662051). This antibody was used for immunohistochemistry on cartilaginous tissue (Vickers et al., 2010; PMID: 20225321).

Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

-20°C,2°C to 8°C

12352203