Anti-Collagen type II [M2139]

Absolute Antibody

Anti-Collagen type II [M2139]

7

Epitope localization of the original format of this antibody (mouse IgG2b) was conducted using ELISA (Schulte et al., 1998; PMID: 9430695). this antibody, in combination with CIIC1, was utilized to induce collagen type II (Col II) antibody-induced arthritis in vivo in mice (Nandakumar et al., 2003; PMID: 14578183). This combination has later been shown to be potently arthritogenic in vivo, affecting cartilage matrix integrity in cartilage explant cultures, impairing synthesis of new matrix, and disrupting collagen fibril formation in chondrocyte cultures (Croxford et al., 2013; PMID: 23239042). Moreover, this antibody was employed in a study to investigate whether mAb CIIF4, when given in combination with this antibody and CIIC1, can prevent cartilage damage in bovine cartilage explants in vitro (Croxford et al., 2010; PMID: 20662051). The original version of this antibody was utilized to examine the production of collagen II with IHC staining (Gharravi et al., 2016; PMID: 26254592) and in the immunohistochemical staining of Col II (Soetjahjo et al., 2018; PMID: n/a).

ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203