Anti-Complement C3 [1H8]

Absolute Antibody

Anti-Complement C3 [1H8]

7

To show that a monoclonal antibody directed against the merozoite and human polyclonal IgG from merozoite vaccine recipients enhanced RBC invasion in a complement-dependent manner, immunofluorescence was performed on merozoites with RBC using the mouse version of this antibody (Biryukov et al, 2016; pmid:27333049). Flow cytometry was performed on intact and lysed PNH erythrocytes using the mouse version of this antibody to analyze the deposition of C3 (Lindorfer et al, 2010; pmid:20068220). While studying the role of the complement system in childhood malaria, immunoprecipitation was performed on erythrocytes using the mouse version of this antibody. Furthermore, a western blot was performed on erythrocytes using this antibody (Pawluczkowycz et al, 2007; pmid:17911641). To examine the ability of several commercially available phycoerythrin (PE)-labeled anti-Mouse IgG antibodies to bind to B cells opsonized in milieu which allow or preclude complement activation, flow cytometry was performed on Raji cells, Z138C cells or CLL patient B cells using the mouse version of this antibody. Furthermore, Raji cells, Z138C cells or CLL patient B cells were stained for immunofluorescence using this antibody (Beum et al, 2004; pmid:15604014).

Flow Cytometry, ImmunoFluorescence, Western Blot

WB; IF; FC

Research Use Only

Complement C3

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203