Figure 4. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562). Connexin 45/GJA7 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Connexin 45/GJA7 Antibody (A08562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (A08562). Connexin 45/GJA7 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Connexin 45/GJA7 Antibody (A08562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Connexin 45/GJA7/GJC1 Antibody Picoband(r)
A08562-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGJC1
Overview
- SupplierBoster Bio
- Product NameAnti-Connexin 45/GJA7/GJC1 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID10052
- Target nameGJC1
- Target descriptiongap junction protein gamma 1
- Target synonymsCX45, GJA7, gap junction gamma-1 protein, CTC-296K1.4, connexin-45, gap junction alpha-7 protein, gap junction protein, gamma 1, 45kDa
- HostRabbit
- IsotypeIgG
- Protein IDP36383
- Protein NameGap junction gamma-1 protein
- Scientific DescriptionBoster Bio Anti-Connexin 45/GJA7/GJC1 Antibody Picoband® catalog # A08562. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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