Anti-Covid-19 & SARS-CoV Nucleocapsid [1C7C7]

Absolute Antibody

Anti-Covid-19 & SARS-CoV Nucleocapsid [1C7C7]

9

This antibody specifically targets an epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein and does not cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E.

The antibody 1C7C7 detects SARS-CoV-2 and SARS-CoV-1 and can be used in affinity binding assays, flow cytometry, immunocytochemistry, and western blotting. Its effectiveness in detecting the nucleocapsid of the novel coronavirus causing COVID-19 was confirmed in multiple studies. For instance, it was used to analyze SARS-CoV-2 nucleoprotein extracted from infected cells via Western blot (Hoagland et al., 2020) ,and similarly by Blanco-Melo et al. in a study on imbalanced host response to SARS-CoV-2 (2020; pmid: 32416070). Another group has used this clone to stain infected cells fixed with 10% neutral formalin during a neutralization assay (Piepenbrink et al., 2020). Daniloski et al. performed similar staining procedure with 1C7C7 for a trans-complementation assay (2020; pmid: 33147445). Finally, this antibody was also used to stain SARs-CoV-2 infected cells in a study on in vitro susceptibility to SARS-CoV-2 infection (Dobrindt et al., 2020; pmid: 32995783).

Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

1C7C7

N

nucleocapsid phosphoprotein

GU280_gp10; nucleocapsid phosphoprotein

Mouse

Nucleoprotein

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203