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Immunohistochemical staining of human stomach shows distinct granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows distinct granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows distinct granular cytoplasmic positivity in glandular cells.

Anti-COX5B Antibody

HPA034517
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCOX5B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-COX5B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1329
  • Target name
    COX5B
  • Target description
    cytochrome c oxidase subunit 5B
  • Target synonyms
    COXVB; cytochrome c oxidase polypeptide VB, mitochondrial; cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase subunit Vb; epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10606
  • Protein Name
    Cytochrome c oxidase subunit 5B, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COX5B using anti-COX5B antibody (A06090-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human MCF-7 whole cell lysates. Lane 7: human HCCT tissue lysates, Lane 8: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX5B antigen affinity purified polyclonal antibody (Catalog # A06090-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX5B at approximately 15 kDa. The expected band size for COX5B is at 15 kDa.
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