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Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in reaction center cells.

Anti-CPB2 Antibody

HPA004146
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCPB2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CPB2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1361
  • Target name
    CPB2
  • Target description
    carboxypeptidase B2
  • Target synonyms
    CPU, PCPB, TAFI, carboxypeptidase B2, carboxypeptidase B-like protein, carboxypeptidase B2 (plasma), carboxypeptidase B2 (plasma, carboxypeptidase U), carboxypeptidase R, thrombin-activable fibrinolysis inhibitor, thrombin-activatable fibrinolysis inhibitor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96IY4
  • Protein Name
    Carboxypeptidase B2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CPB2 using anti-CPB2 antibody (PB9997). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPB2 antigen affinity purified polyclonal antibody (Catalog # PB9997) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPB2 at approximately 48 kDa, 60 kDa. The expected band size for CPB2 is at 48 kDa.
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