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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

Anti-CPEB4 Antibody

HPA038394
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCPEB4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CPEB4
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80315
  • Target name
    CPEB4
  • Target description
    cytoplasmic polyadenylation element binding protein 4
  • Target synonyms
    CPE-BP4, hCPEB-4, cytoplasmic polyadenylation element-binding protein 4, CPE-binding protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ17RY0
  • Protein Name
    Cytoplasmic polyadenylation element-binding protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Immunofluorescence staining of Hela cells with CSB-PA618177LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of CPEB4 using anti-CPEB4 antibody (A06139-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPEB4 antigen affinity purified polyclonal antibody (Catalog # A06139-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPEB4 at approximately 90 kDa. The expected band size for CPEB4 is at 80 kDa.
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