Transfected HEK293 cells with GFP-Cas9-SA fusion protein were stained with Anti-Cas9 from Staphylococcus Aureus Antibody. GFP is expressed in the transfected cells (green). GFP-Cas9 fusion protein is stained with Anti-Cas9 from Staphylococcus Aureus Antibody (1:1,000 | red). Only transfected cells are reactive with Anti-Cas9 from Staphylococcus Aureus Antibody, which appear in a yellow-orange color (merge). Nuclei are visualized in blue with Hoechst staining.
![Immunofluorescent analysis of HEK293 cells transfected with a construct of green fluorescent protein (GFP) fused with the C-terminal sequence (aa. 803-1053) of <i>S. aureus</i> Cas9. Cells were stained with Anti-Cas9 Antibody [6F7] (A85319) in red, and this combined with the expressed GFP gives a green signal. The two signals overlap as expected. The nuclei of both transfected and untransfected cells are revealed by the DAPI DNA stain.](https://cdn.antibodies.com/image/catalog/85/A85319_2.jpg "Immunofluorescent analysis of HEK293 cells transfected with a construct of green fluorescent protein (GFP) fused with the C-terminal sequence (aa. 803-1053) of <i>S. aureus</i> Cas9. Cells were stained with Anti-Cas9 Antibody [6F7] (A85319) in red, and this combined with the expressed GFP gives a green signal. The two signals overlap as expected. The nuclei of both transfected and untransfected cells are revealed by the DAPI DNA stain.")
![Western blot analysis of HEK293 cell lysates using Anti-Cas9 Antibody [6F7] (A85319), in green. The lanes contain samples of: <b>[1]</b> Protein standards, in red, <b>[2]</b> non-transfected cells, and <b>[3]</b> cells transfected with a GFP-CAS9 fusion construct, encoding the C-terminal (803-1053) amino acids of S. aureus CAS9. The strong band at about 53 kDa corresponds to the GFP-CAS9 fusion protein.](https://cdn.antibodies.com/image/catalog/85/A85319_3.jpg "Western blot analysis of HEK293 cell lysates using Anti-Cas9 Antibody [6F7] (A85319), in green. The lanes contain samples of: <b>[1]</b> Protein standards, in red, <b>[2]</b> non-transfected cells, and <b>[3]</b> cells transfected with a GFP-CAS9 fusion construct, encoding the C-terminal (803-1053) amino acids of S. aureus CAS9. The strong band at about 53 kDa corresponds to the GFP-CAS9 fusion protein.")
Transfected HEK293 cells with GFP-Cas9-SA fusion protein were stained with Anti-Cas9 from Staphylococcus Aureus Antibody. GFP is expressed in the transfected cells (green). GFP-Cas9 fusion protein is stained with Anti-Cas9 from Staphylococcus Aureus Antibody (1:1,000 | red). Only transfected cells are reactive with Anti-Cas9 from Staphylococcus Aureus Antibody, which appear in a yellow-orange color (merge). Nuclei are visualized in blue with Hoechst staining.
Anti-CRISPR-Cas9 Antibody [6F7]
A85319
Product group Antibodies
Overview
- SupplierAntibodies.com
- Product NameAnti-CRISPR-Cas9 Antibody [6F7]
- Delivery Days Customer7
- UNSPSC12352203