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ProductsBack/Anti-Ctip2 [25B6]

Anti-Ctip2 [25B6]

Ab00616-23.0
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBCL11B
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Ctip2 [25B6]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody detects two bands corresponding to Ctip2, a protein highly expressed in brain and malignant T-cells from patients with adult T-cell leukemia/lymphoma.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF; IHC-Fr; IHC-P; WB; IP; FC; CHIP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    25B6
  • Gene ID64919
  • Target name
    BCL11B
  • Target description
    BAF chromatin remodeling complex subunit BCL11B
  • Target synonyms
    ATL1; ATL1-alpha; ATL1-beta; ATL1-delta; ATL1-gamma; B cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B (zinc finger protein); B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B; BCL-11B; BCL11B, BAF complex component; BCL11B/TRDC fusion; COUP-TF-interacting protein 2; CTIP2; CTIP-2; hRit1; hRIT1-alpha; IDDFSTA; IMD49; radiation-induced tumor suppressor gene 1 protein; RIT1; SMARCM2; zinc finger protein hRit1 alpha; ZNF856B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0K0
  • Protein Name
    B-cell lymphoma/leukemia 11B
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse, Rat, Guinea pig, Human, Zebrafish, Cynomolgus Monkey
  • Reactivity Supplier Note
    Human fusion protein.9
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ctip2/BCL11B using anti-Ctip2/BCL11B antibody (A01485-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ctip2/BCL11B antigen affinity purified polyclonal antibody (Catalog # A01485-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ctip2/BCL11B at approximately 130 kDa. The expected band size for Ctip2/BCL11B is at 96 kDa.
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