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Immunohistochemical staining of human esophagus shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows moderate nuclear positivity in squamous epithelial cells.

Anti-CTNNBL1 Antibody

HPA027907
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCTNNBL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CTNNBL1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56259
  • Target name
    CTNNBL1
  • Target description
    catenin beta like 1
  • Target synonyms
    C20orf33, IMD99, NAP, P14L, PP8304, dJ633O20.1, beta-catenin-like protein 1, nuclear associated protein, testis development protein NYD-SP19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYA6
  • Protein Name
    Beta-catenin-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CTNNBL1 using anti-CTNNBL1 antibody (A05286-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNBL1 antigen affinity purified polyclonal antibody (A05286-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNBL1 at approximately 65 kDa. The expected band size for CTNNBL1 is at 65 kDa.
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