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Figure 1. Western blotting validation for Anti-CXADR Antibody A02747 Western blot (WB) analysis of CXADR polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-CXADR Antibody A02747 Western blot (WB) analysis of CXADR polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-CXADR Antibody A02747 Western blot (WB) analysis of CXADR polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:H9C2 cell lysate Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Anti-CXADR Antibody

A02747
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCXADR
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CXADR Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID1525
  • Target name
    CXADR
  • Target description
    CXADR cell adhesion molecule
  • Target synonyms
    CAR, CAR4/6, HCAR, coxsackievirus and adenovirus receptor, 46 kD coxsackievirus and adenovirus receptor (CAR) protein, CVB3-binding protein, CXADR Ig-like cell adhesion molecule, HCVADR, coxsackie virus and adenovirus receptor, coxsackievirus B-adenovirus receptor
  • Host
    Rabbit
  • Protein IDP78310
  • Protein Name
    Coxsackievirus and adenovirus receptor
  • Scientific Description
    Boster Bio Anti-CXADR Antibody catalog # A02747. Tested in WB,ICC applications. This antibody reacts with Human,Mouse,Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CXADR using anti-CXADR antibody (A02747-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXADR antigen affinity purified polyclonal antibody (Catalog # A02747-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXADR at approximately 45 kDa. The expected band size for CXADR is at 40 kDa.
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