Anti-CXCR3 [9C5]

Absolute Antibody

Anti-CXCR3 [9C5]

9

This antibody strongly binds human, mouse and marmoset CXCR3. Epitope mapping revealed that this antibody binds a peptide (FSSSYDYGENESDSCCTSPPCP) which corresponds to amino acid position 23-44 of the human CXCR3.

The binding of this antibody with CXCR3 from various species was assessed by flow cytometry. It was shown to recognize LI.2 cells transfected with mouse CXCR3 (mCXCR3) or human CXCR3 (hCXCR3), but not untransfected LI.2 cells or cells transfected with other chemokine receptors (i.e. mCXCR2) in flow cytometry. Epitope mapping of this antibody was performed using ELISA. To access the in vivo effect, various concentrations of the antibody were injected intraveneously in a mice. It was seen that the original 9C4, mouse IgG1 version, completely depleted CD4+CXCR3+, CD8+/CXCR3+ and B2207CXCR3+ cells at a concentration as low as 2 mg/kg (WO2014186842). Chimeric antibodies derived from 9C5 called 9C5-3MFc and 9C5-Fc-KO and humanized verion are also available on request.

Flow Cytometry, ELISA

Research Use Only

Monoclonal

9C5

CXCR3

C-X-C motif chemokine receptor 3

CD182; CD183; chemokine (C-X-C motif) receptor 3; chemokine receptor 3; CKR-L2; CMKAR3; C-X-C chemokine receptor type 3; G protein-coupled receptor 9; GPR9; interferon-inducible protein 10 receptor; IP-10 receptor; IP10-R; Mig receptor; MigR; Mig-R

Mouse

IgG1

C-X-C chemokine receptor type 3

Human, Mouse, Primate

-20°C,2°C to 8°C

12352203