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Anti-cytochrome C [R1D3]

AB03812-1.1
Absolute Antibody
ApplicationsImmunoCytoChemistry
Product group Antibodies
TargetCycs
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-cytochrome C [R1D3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody was employed to identify alternative conformations of cyt c in cells under oxidant- or senescence-induced challenge as confirmed by immunocytochemistry (Tomasina et al., 2022; PMID: 36378644).
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    R1D3
  • Gene ID13063
  • Target name
    Cycs
  • Target description
    cytochrome c, somatic
  • Target synonyms
    cytochrome c, somatic
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP62897
  • Protein Name
    Cytochrome c, somatic
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytochrome C using anti-Cytochrome C antibody (A03529). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome C antigen affinity purified polyclonal antibody (Catalog # A03529) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome C at approximately 14 kDa. The expected band size for Cytochrome C is at 12 kDa.
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