Figure 2. IHC analysis of Cytoglobin using anti-Cytoglobin antibody (A01197). Cytoglobin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Cytoglobin Antibody (A01197) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of Cytoglobin using anti-Cytoglobin antibody (A01197). Cytoglobin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Cytoglobin Antibody (A01197) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Cytoglobin/CYGB Antibody Picoband(r)
A01197-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCYGB
Overview
- SupplierBoster Bio
- Product NameAnti-Cytoglobin/CYGB Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID114757
- Target nameCYGB
- Target descriptioncytoglobin
- Target synonymscytoglobin; HGB; histoglobin; STAP; stellate cell activation-associated protein
- HostRabbit
- IsotypeIgG
- Protein IDQ8WWM9
- Protein NameCytoglobin
- Scientific DescriptionBoster Bio Anti-Cytoglobin/CYGB Antibody Picoband® catalog # A01197. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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