Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]

Absolute Antibody

Anti-Cytotoxin 1, 3, 7 [TPL0027_01_F7]

7

TPL0027_01_F7 antibody recognizes cytotoxins 1, 3 and 7, which are some of the major toxins in the venom of the black-necked spitting cobra (Naja nigricollis).

TPL0027_01_F7 antibody has high affinity to the cytotoxins 1, 3 and 7. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibodys high affinity was confirmed by its ability to bind the target proteins in a venom fraction even at 1:32,000 dilution (3.125 10^-2) microg/mL (Juergensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites.

ELISA

ELISA

Research Use Only

Monoclonal

TPL0027_01_F7

Mouse

IgM

This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Naja nigricollis

The antibody was raised using IONTAS phage display library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind a toxin, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind.

-20°C,2°C to 8°C

12352203