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ProductsBack/Anti-D-Dimer [DD-12B4]

Anti-D-Dimer [DD-12B4]

AB04006-23.0-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetFGA
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-D-Dimer [DD-12B4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The initial binding characterization of this antibody to human D-dimer fragment was done using ELISA. This antibody was also used in the generation of a kit for detection of blood coagulation (CN113717285).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DD-12B4
  • Gene ID2243
  • Target name
    FGA
  • Target description
    fibrinogen alpha chain
  • Target synonyms
    AMYLD2, Fib2, fibrinogen alpha chain, fibrinogen, A alpha polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02671
  • Protein Name
    Fibrinogen alpha chain
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FGA using anti-FGA antibody (A00816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGA antigen affinity purified polyclonal antibody (Catalog # A00816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGA at approximately 95KD. The expected band size for FGA is at 95KD.
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