Anti-D-Lactyllysine Rabbit mAb

PTM BIO

Anti-D-Lactyllysine Rabbit mAb

5

Anti-D-Lactyllysine Rabbit mAb detects proteins with D-lactylated lysine residues, with no cross-reactivity to its isomers L-lactyllysine or N-epsilon-(carboxyethyl)-lysine (Kce). This pan antibody recognizes D-lactylated lysine regardless of the surrounding amino acid sequences.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry

WB, ICC/IF, IP

Antibody

Research Use Only

Monoclonal

CAPTM-O0804

Unconjugated

Rabbit

IgG

D-lactyllysine (KD-la) refers to the modification of lysine residues in proteins by the addition of D-lactate.dues in proteins. This modification was first reported by Zhang et al. in 2024, with the findings published in Nature Chemical Biology. KD-la forms through a non-enzymatic reaction between proteins and S-D-lactoylglutathione (LGSH), which is a byproduct of the glyoxalase pathway. This pathway involves two key enzymes: glyoxalase 1 (GLO1) and glyoxalase 2 (GLO2). GLO1 conjugates methylglyoxal (MGO), a glycolysis byproduct, with glutathione to form LGSH, which is hydrolyzed by GLO2 to produce D-lactate while regenerating cellular glutathione.In humans, L-lactate is a common metabolic product that plays a key role in energy metabolism, especially during muscle activity. In contrast, D-lactate is not a typical product of human metabolism but is mainly produced by microorganisms such as certain bacteria and yeasts during fermentation. The potential harm of D-lactate mainly lies in its accumulation under specific conditions, such as intestinal microbiota dysbiosis, short bowel syndrome, or certain disease states. Excessive production of D-lactate can lead to D-lactic acidosis, characterized by symptoms of acidosis (low blood bicarbonate levels, decreased blood pH, and hyperuricemia), and in severe cases, neurological symptoms like seizures, ataxia, and altered consciousness.

Stable for 12 months from date of receipt/reconstitution.

Human

Human

Protein A purified

Store at -20°C. Avoid freeze/thaw cycles.

12352203