Figure 1. Western blot analysis of DAI/Zbp1 using anti-DAI/Zbp1 antibody (A04739-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAI/Zbp1 antigen affinity purified polyclonal antibody (Catalog # A04739-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAI/Zbp1 at approximately 63 kDa. The expected band size for DAI/Zbp1 is at 63 kDa.
Figure 1. Western blot analysis of DAI/Zbp1 using anti-DAI/Zbp1 antibody (A04739-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAI/Zbp1 antigen affinity purified polyclonal antibody (Catalog # A04739-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAI/Zbp1 at approximately 63 kDa. The expected band size for DAI/Zbp1 is at 63 kDa.
Anti-DAI/Zbp1 Antibody Picoband(r)
A04739-3-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetZbp1
Overview
- SupplierBoster Bio
- Product NameAnti-DAI/Zbp1 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID58203
- Target nameZbp1
- Target descriptionZ-DNA binding protein 1
- Target synonyms2010010H03Rik, Dai, Dlm1, mZaDLM, Z-DNA-binding protein 1, DNA-dependent activator of IFN-regulatory factors, tumor stroma and activated macrophage protein DLM-1
- HostRabbit
- IsotypeIgG
- Scientific DescriptionBoster Bio Anti-DAI/Zbp1 Antibody Picoband® catalog # A04739-3. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203