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Immunofluorescence staining of rat caudate putamen shows moderate positivity in dopaminergic fibers.
Immunofluorescence staining of rat caudate putamen shows moderate positivity in dopaminergic fibers.
Immunofluorescence staining of rat caudate putamen shows moderate positivity in dopaminergic fibers.

Anti-DAT Antibody

AMAB91125
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC6A3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DAT
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL3123
  • Conjugate
    Unconjugated
  • Gene ID6531
  • Target name
    SLC6A3
  • Target description
    solute carrier family 6 member 3
  • Target synonyms
    DAT, DAT1, PKDYS, PKDYS1, sodium-dependent dopamine transporter, DA transporter, dopamine transporter 1, solute carrier family 6 (neurotransmitter transporter), member 3, solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ01959
  • Protein Name
    Sodium-dependent dopamine transporter
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Dopamine Transporter/SLC6A3 using anti-Dopamine Transporter/SLC6A3 antibody (A00369-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dopamine Transporter/SLC6A3 antigen affinity purified polyclonal antibody (Catalog # A00369-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dopamine Transporter/SLC6A3 at approximately 68 kDa. The expected band size for Dopamine Transporter/SLC6A3 is at 68 kDa.
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