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Immunohistochemistry analysis in human kidney and pancreas tissues using Anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and pancreas tissues using Anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and pancreas tissues using Anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.

Anti-DBT Antibody

HPA026533
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDBT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DBT Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1629
  • Target name
    DBT
  • Target description
    dihydrolipoamide branched chain transacylase E2
  • Target synonyms
    BCATE2, BCKAD-E2, BCKADE2, BCKDH-E2, BCOADC-E2, E2, E2B, lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, BCKAD E2 subunit, E2 component of branched chain alpha-keto acid dehydrogenase complex, branched chain 2-oxo-acid dehydrogenase complex component E2, branched chain acyltransferase, E2 component, branched-chain alpha-keto acid dehydrogenase complex component E2, dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, dihydrolipoyl transacylase, dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex, mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11182
  • Protein Name
    Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DBT using anti-DBT antibody (A01145-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBT antigen affinity purified polyclonal antibody (A01145-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBT at approximately 53 kDa. The expected band size for DBT is at 53 kDa.
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