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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules and glomeruli.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules and glomeruli.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules and glomeruli.

Anti-DCAF13 Antibody

HPA044504
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDCAF13
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DCAF13 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25879
  • Target name
    DCAF13
  • Target description
    DDB1 and CUL4 associated factor 13
  • Target synonyms
    GM83, HSPC064, Sof1, WDSOF1, DDB1- and CUL4-associated factor 13, WD repeat and SOF domain-containing protein 1, WD repeats and SOF1 domain containing
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NV06
  • Protein Name
    DDB1- and CUL4-associated factor 13
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of DCAF13 using anti-DCAF13 antibody (M13516). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whoel cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF13 antigen affinity purified monoclonal antibody (M13516) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCAF13 at approximately 48 kDa. The expected band size for DCAF13 is at 51 kDa.
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