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Anti-DDB1 Antibody

R1706-21
HUABIO
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
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Product group Antibodies
Price on request
100 ul
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Overview

  • Supplier
    HUABIO
  • Product Name
    Anti-DDB1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB,IF-Cell,IHC-P,FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/mL.
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃ Avoid repeated freeze / thaw cycles.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DDB1 using anti-DDB1 antibody (PB9578). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB1 antigen affinity purified polyclonal antibody (Catalog # PB9578) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB1 at approximately 127 kDa. The expected band size for DDB1 is at 127 kDa.
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