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Immunofluorescent staining of human cell line BJ shows localization to actin filaments.
Immunofluorescent staining of human cell line BJ shows localization to actin filaments.
Immunofluorescent staining of human cell line BJ shows localization to actin filaments.

Anti-DDR2 Antibody

HPA070112
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetDDR2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DDR2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4921
  • Target name
    DDR2
  • Target description
    discoidin domain receptor tyrosine kinase 2
  • Target synonyms
    MIG20a, NTRKR3, TKT, TYRO10, WRCN, discoidin domain-containing receptor 2, CD167 antigen-like family member B, cell migration-inducing protein 20, discoidin domain receptor 2, discoidin domain receptor family, member 2, discoidin domain-containing receptor tyrosine kinase 2, hydroxyaryl-protein kinase, migration-inducing gene 16 protein, neurotrophic tyrosine kinase receptor related 3, receptor protein-tyrosine kinase TKT, tyrosine-protein kinase TYRO10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16832
  • Protein Name
    Discoidin domain-containing receptor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DDR2 using anti-DDR2 antibody (A01698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody (Catalog # A01698-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR2 at approximately 120 kDa. The expected band size for DDR2 is at 98 kDa.
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