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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.

Anti-DDX6 Antibody

HPA024201
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDDX6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DDX6 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1656
  • Target name
    DDX6
  • Target description
    DEAD-box helicase 6
  • Target synonyms
    HLR2, IDDILF, P54, RCK, Rck/p54, probable ATP-dependent RNA helicase DDX6, putative ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box helicase 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD box protein 6, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), oncogene RCK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26196
  • Protein Name
    Probable ATP-dependent RNA helicase DDX6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DDX6 using anti-DDX6 antibody (A03826-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates, Lane 9: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX6 antigen affinity purified polyclonal antibody (Catalog # A03826-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX6 at approximately 54KD. The expected band size for DDX6 is at 54KD.
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