Anti-Dengue virus envelope protein [C9]

Absolute Antibody

Anti-Dengue virus envelope protein [C9]

9

This antibody is specific for the EDIII protein of the dengue virus. The dengue virus causes the disease known as dengue fever.

To determine the specificity and DENV cross-reactivity of the IgG1 version of this antibody an ELISA was performed on EDIII, this antibody had strong binding for EDIII from DENV2 and to a lesser extent with EDIII from DENV1 and DENV3. Furthermore, immunofluorescence was performed on uninfected BHK-21 cells and cells infected with DENV2 using the IgG1 version of this antibody, it showed clear fluorescence in the cytoplasm of infected cells. Finally, a plaque reduction neutralization test was performed using the IgG1 version of this antibody, this antibody showed a 25% reduction of plaque formation. This antibody is a chimeric antibody with mouse Vkappa/human Ckappa and mouse VH/human CH segments (Moreland et al, 2012; pmid:22489114).

ImmunoFluorescence, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

C9

POLY

polyprotein

DV1_gp1; polyprotein; polyprotein gene

Mouse

IgG1

Genome polyprotein

Virus

-20°C,2°C to 8°C

12352203