Anti-Dengue virus envelope protein [C9]

Absolute Antibody

Anti-Dengue virus envelope protein [C9]

7

To determine the specificity and DENV cross-reactivity of the IgG1 version of this antibody an ELISA was performed on EDIII, this antibody had strong binding for EDIII from DENV2 and to a lesser extent with EDIII from DENV1 and DENV3. Furthermore, immunofluorescence was performed on uninfected BHK-21 cells and cells infected with DENV2 using the IgG1 version of this antibody, it showed clear fluorescence in the cytoplasm of infected cells. Finally, a plaque reduction neutralization test was performed using the IgG1 version of this antibody, this antibody showed a 25% reduction of plaque formation. This antibody is a chimeric antibody with mouse Vkappa/human Ckappa and mouse VH/human CH segments (Moreland et al, 2012; pmid:22489114).

ImmunoFluorescence, ELISA, Neutralisation/Blocking

ELISA; neutralization; IF

Research Use Only

Genome polyprotein

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203