Anti-Deoxynivalenol [AbDON01]

Absolute Antibody

Anti-Deoxynivalenol [AbDON01]

7

This antibody is specific for mycotoxin deoxynivalenol (DON). This antibody also shows reactivity with other trichothecene mycotoxins like 15-AC-deoxynivalenol, HT-2 toxin, T-2 triol, nivalenol (NIV) and T-2 toxin/tri-acetyl-DON (Tri-Ac-DON) but it does n

The binding characterization of the scFv antibody and the whole IgG1 antibody was done using non-competitive indirect ELISA. To check the cross reactivity of the scFv version and whole IgG version with other members of trichothecene mycotoxins, competitive direct ELISA and biolayer interferometry (BLI) was used (Maragos et al., 2012).

ELISA

ELISA

Research Use Only

Monoclonal

AbDON01

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.

Fungus

Fusarium species

The original antibody was generated by cloning the variable light and heavy chain genes of the anti-DOM hybridoma were assembled into the scFv gene using a linker sequence (Gly4Ser)3 by over-lapping PCR. PCR amplified products were purified, treated with SfiI and NotI and cloned into phagemid expression pDisplay-2TM . The plasmid was used to transform E.coli HB2151 host cells and the soluble scFv was generated.

-20°C,2°C to 8°C

12352203