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Anti-DHODH (Center) Antibody

102-23613
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetDHODH
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-DHODH (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB40540
  • Gene ID1723
  • Target name
    DHODH
  • Target description
    dihydroorotate dehydrogenase (quinone)
  • Target synonyms
    DHOdehase, POADS, URA1, dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate oxidase, human complement of yeast URA1
  • Host
    Rabbit
  • Protein IDQ02127
  • Protein Name
    Dihydroorotate dehydrogenase (quinone), mitochondrial
  • Scientific Description
    Rabbit Anti-DHODH(Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DHODH using anti-DHODH antibody (PB10056). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHODH antigen affinity purified polyclonal antibody (Catalog # PB10056) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHODH at approximately 43 kDa. The expected band size for DHODH is at 43 kDa.
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