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Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-DHPS Antibody

HPA014461
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetDHPS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DHPS
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1725
  • Target name
    DHPS
  • Target description
    deoxyhypusine synthase
  • Target synonyms
    deoxyhypusine synthase; DHS; DS; MIG13; migration-inducing gene 13; NEDSSWI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49366
  • Protein Name
    Deoxyhypusine synthase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DHPS/DHS using anti-DHPS/DHS antibody (A02106-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human U937 whole cell lysates, Lane 9: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHPS/DHS antigen affinity purified polyclonal antibody (Catalog # A02106-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHPS/DHS at approximately 41 kDa. The expected band size for DHPS/DHS is at 41 kDa.
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