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Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Anti-DHX15 Antibody

HPA047047
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDHX15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DHX15
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1665
  • Target name
    DHX15
  • Target description
    DEAH-box helicase 15
  • Target synonyms
    ATP-dependent RNA helicase #46; DBP1; DDX15; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15; DEAD/H box-15; DEAH (Asp-Glu-Ala-His) box helicase 15; DEAH (Asp-Glu-Ala-His) box polypeptide 15; DEAH box protein 15; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; PRP43; PRPF43; PrPp43p; putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; RNA helicase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43143
  • Protein Name
    Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: DHX15 antibody at 3.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 91 kDa Observed band size: 91 kDa
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Figure 1. Western blot analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human U251 whole cell lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX15/prp43 antigen affinity purified polyclonal antibody (Catalog # A08140-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX15/prp43 at approximately 91 kDa. The expected band size for DHX15/prp43 is at 91 kDa.
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