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Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows cytoplasmic and membranous positivity in glandular cells.

Anti-DHX29 Antibody

HPA038317
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDHX29
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DHX29 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54505
  • Target name
    DHX29
  • Target description
    DExH-box helicase 29
  • Target synonyms
    DDX29, ATP-dependent RNA helicase DHX29, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 29, DEAH (Asp-Glu-Ala-His) box polypeptide 29, DEAH box protein 29, DEAH-box helicase 29, nucleic acid helicase DDXx
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z478
  • Protein Name
    ATP-dependent RNA helicase DHX29
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DHX29 using anti-DHX29 antibody (A07866). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HT1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX29 antigen affinity purified polyclonal antibody (Catalog # A07866) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX29 at approximately 155 kDa. The expected band size for DHX29 is at 155 kDa.
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