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Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Anti-DHX9 Antibody

HPA055684
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetDHX9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DHX9 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1660
  • Target name
    DHX9
  • Target description
    DExH-box helicase 9
  • Target synonyms
    DDX9, LKP, MRD75, NDH2, NDHII, RHA, ATP-dependent RNA helicase A, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9, DEAH (Asp-Glu-Ala-His) box helicase 9, DEAH (Asp-Glu-Ala-His) box polypeptide 9, DEAH box protein 9, DEAH-box helicase 9, RNA helicase A, leukophysin, nuclear DNA helicase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08211
  • Protein Name
    ATP-dependent RNA helicase A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DHX9 using anti-DHX9 antibody (A02550-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human Raji whole cell lysates, Lane 8: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX9 antigen affinity purified polyclonal antibody (Catalog # A02550-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX9 at approximately 141KD. The expected band size for DHX9 is at 141KD.
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