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Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in sinusoids.
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in sinusoids.
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in sinusoids.

Anti-DIAPH1 Antibody

HPA004916
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDIAPH1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DIAPH1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1729
  • Target name
    DIAPH1
  • Target description
    diaphanous related formin 1
  • Target synonyms
    DFNA1, DIA1, DRF1, LFHL1, SCBMS, hDIA1, mDia1, protein diaphanous homolog 1, mammalian diaphanous related formin 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60610
  • Protein Name
    Protein diaphanous homolog 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DIAPH1 using anti-DIAPH1 antibody (A02308-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIAPH1 antigen affinity purified polyclonal antibody (Catalog # A02308-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DIAPH1 at approximately 150 kDa. The expected band size for DIAPH1 is at 150 kDa.
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