Anti-Diethoxyphosphotyrosine [DepY]

Absolute Antibody

Anti-Diethoxyphosphotyrosine [DepY]

7

This antibody was characterized in order to determine its binding affinity and binding specificity. The original mouse antibody recognized diethoxyphosphotyrosine independent of the amino acid sequence around the modified tyrosine and independent of the identity of the carrier protein or peptide in an ELISA and western blotting assay. The original mouse IgG2a version of this antibody had an IC50 of 3 x 10-9 M in a competition assay with OP tubulin. Kd values measured by BIAcore and OctetRED96 were 10-8 M for OP-peptides and 1 x 10-12 M for OP-proteins. The limit of detection measured on western blots hybridized with 0.14 microg/mL of depY was 0.025 microg of human albumin conjugated to YGGFL-OP (PMID: 29137457).

Western Blot, ELISA, Other Application

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203