Anti-disialoganglioside 2 [7A4]

Absolute Antibody

Anti-disialoganglioside 2 [7A4]

7

7A4 binds to GD2: GD2+ neuroblastoma cell lines and melanomas, small-cell lung carcinomas, rhabdomyosarcomas, Ewings sarcomas, Wilms tumors and retinoblastomas also expressing GD2. GD2- cell lines or GD2- peripheral blood cells are not bound by the antibo

7A4 is able to lyse tumour cells by complement-mediated cytotoxicity (lytic dose ~10 ng) and can perform in vitro direct binding of labelled mAb to living and glutaraldehyde-fixed cells. Shows strong binding to all neuroblastomas tested in addition to other GD2-expressing cells such as melanomas, small-cell lung carcinomas, rhabdomyosarcomas, Ewings sarcomas, Wilms tumors and retinoblastomas. TLC analysis can be used to confirm the antibody target antigen. The 7A4 scFv possesses the same specificity as the full-length antibody but slightly weaker binding affinity. The scFv has been used in IF studies (PMID: 9309424).

ImmunoFluorescence, ELISA, ImmunoHistoChemistry, Other Application, RadioImmunoAssay

ELISA; RIA; TLC; IF; depletion; IHC

Research Use Only

Monoclonal

7A4

Human

IgG1

Human

Human

7A4 was generated by hyperimmunizing mice with recombinant gamma-interferon-treated LAN-1 neuroblastoma cells.

-20°C,2°C to 8°C

12352203