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Anti-DKK3 Antibody

144-65538
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetDKK3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-DKK3 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID27122
  • Target name
    DKK3
  • Target description
    dickkopf WNT signaling pathway inhibitor 3
  • Target synonyms
    dickkopf 3 homolog; dickkopf homolog 3; dickkopf-3; dickkopf-related protein 3; dkk-3; hDkk-3; regulated in glioma; REIC; RIG; RIG-like 5-6; RIG-like 7-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBP4
  • Protein Name
    Dickkopf-related protein 3
  • Scientific Description
    DKK3 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DKK3/RIG using anti-DKK3/RIG antibody (A03140). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK3/RIG antigen affinity purified polyclonal antibody (Catalog # A03140) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK3/RIG at approximately 22 kDa. The expected band size for DKK3/RIG is at 12 kDa.
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with DKK3 Polyclonal Antibody (bs-2688R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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