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Western blot analysis of human DLK1 using anti-DLK1 (human), pAb (Prod. No. AG-25A-0091) at 1: 2,000 dilution. A. 1. Human DLK1 (Fc protein). 2. Transfected human DLK1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 c
Western blot analysis of human DLK1 using anti-DLK1 (human), pAb (Prod. No. AG-25A-0091) at 1: 2,000 dilution. A. 1. Human DLK1 (Fc protein). 2. Transfected human DLK1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 c
Western blot analysis of human DLK1 using anti-DLK1 (human), pAb (Prod. No. AG-25A-0091) at 1: 2,000 dilution. A. 1. Human DLK1 (Fc protein). 2. Transfected human DLK1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 c

anti-DLK1 (human), pAb

AG-25A-0091
AdipoGen Life Sciences
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetDLK1
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-DLK1 (human), pAb
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID8788
  • Target name
    DLK1
  • Target description
    delta like non-canonical Notch ligand 1
  • Target synonyms
    Delta1; delta-like 1 homolog; DLK; DLK-1; FA1; fetal antigen 1; pG2; preadipocyte factor 1; PREF1; Pref-1; protein delta homolog 1; secredeltin; ZOG
  • Host
    Rat
  • Protein IDP80370
  • Protein Name
    Protein delta homolog 1
  • Scientific Description
    Polyclonal Antibody. Recognizes human DLK1. Detects a band of ~42kDa by Western blot. Source: Rat. Applications: ELISA, FACS, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-alpha converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion. - The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-alpha converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DLK-1/DLK1 using anti-DLK-1/DLK1 antibody (A00513-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLK-1/DLK1 antigen affinity purified polyclonal antibody (Catalog # A00513-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLK-1/DLK1 at approximately 50 kDa. The expected band size for DLK-1/DLK1 is at 50 kDa.
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