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Anti-DNA topoisomerase 1 [5C2]

AB03245-1.1-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetTOP1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-DNA topoisomerase 1 [5C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding characterization of this antibody was done using ELISA (PMID: 18062792).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C2
  • Gene ID7150
  • Target name
    TOP1
  • Target description
    DNA topoisomerase I
  • Target synonyms
    TOPI, DNA topoisomerase 1, Scl-70 antigen, topoisomerase (DNA) I, type I DNA topoisomerase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP11387
  • Protein Name
    DNA topoisomerase 1
  • Scientific Description
    This full-length chimeric mouse antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Topoisomerase I using anti-Topoisomerase I antibody (A00434-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Topoisomerase I antigen affinity purified polyclonal antibody (Catalog # A00434-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Topoisomerase I at approximately 100 kDa. The expected band size for Topoisomerase I is at 91 kDa.
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