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Anti-DNAJC11 (N-term) Antibody

102-20499
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDNAJC11
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-DNAJC11 (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB23873
  • Gene ID55735
  • Target name
    DNAJC11
  • Target description
    DnaJ heat shock protein family (Hsp40) member C11
  • Target synonyms
    dJ126A5.1, dnaJ homolog subfamily C member 11, DnaJ (Hsp40) homolog, subfamily C, member 11, novel DnaJ domain-containing protein
  • Host
    Rabbit
  • Protein IDQ2NL21
  • Protein Name
    DnaJ homolog subfamily C member 11
  • Scientific Description
    Rabbit Anti-DNAJC11 (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DNAJC11 using anti-DNAJC11 antibody (A11307-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC11 antigen affinity purified polyclonal antibody (Catalog # A11307-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC11 at approximately 63 kDa. The expected band size for DNAJC11 is at 63 kDa.
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