Anti-DNA/RNA G-quadruplex [BG4]

Absolute Antibody

Anti-DNA/RNA G-quadruplex [BG4]

7

This antibody has been shown (by ELISA) to bind selectively to G-quadruplex forming DNA and RNA structures. Please that Fab-based versions as well as scFvs, with and without Flag-tag are available, which needs to be considered when replicating published methods. Tsai et al. (2022 et al. PMID: 36440760), report similar activtiy between whole-antibody and scFv-based versions of BG4. Weldon et al. (2016, PMID: 27820800) used Ab00174-1.1 to identify G-quadruplexes in long functional RNAs using an electrophoretic mobility shift assay (EMSA). The mouse IgG1 version of BG4 was shown to have weak cross-reactivity with iMotif structures (2018, PMID: 29686376). iMotif structures can be detected selectively using our anti-iMotif antibody Ab01462.

ImmunoFluorescence, ELISA, Other Application

Research Use Only

This chimeric mouse antibody Fab-fragment was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. It does not have a FLAG-tag, but a His-tag, which may be used for detection. Alternatively, secondary antibodies raised against murine Fab-fragments, or murine lambda light-chain can be used.

-20°C,2°C to 8°C

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