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Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line CACO-2 shows localization to nuclear speckles.

Anti-DNMT3L Antibody

HPA055234
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetDNMT3L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DNMT3L
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29947
  • Target name
    DNMT3L
  • Target description
    DNA methyltransferase 3 like
  • Target synonyms
    DNA (cytosine-5)-methyltransferase 3-like, DNA (cytosine-5-)-methyltransferase 3-like, cytosine-5-methyltransferase 3-like protein, human cytosine-5-methyltransferase 3-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJW3
  • Protein Name
    DNA (cytosine-5)-methyltransferase 3-like
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DNMT3L using anti-DNMT3L antibody (A04214-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3L antigen affinity purified polyclonal antibody (Catalog # A04214-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3L at approximately 44 kDa. The expected band size for DNMT3L is at 44 kDa.
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