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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Anti-DNMT3L Antibody

HPA066780
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDNMT3L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DNMT3L
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29947
  • Target name
    DNMT3L
  • Target description
    DNA methyltransferase 3 like
  • Target synonyms
    DNA (cytosine-5)-methyltransferase 3-like, DNA (cytosine-5-)-methyltransferase 3-like, cytosine-5-methyltransferase 3-like protein, human cytosine-5-methyltransferase 3-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJW3
  • Protein Name
    DNA (cytosine-5)-methyltransferase 3-like
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DNMT3L using anti-DNMT3L antibody (A04214-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3L antigen affinity purified polyclonal antibody (Catalog # A04214-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3L at approximately 44 kDa. The expected band size for DNMT3L is at 44 kDa.
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