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Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.

Anti-DNTT Antibody

HPA036532
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetDNTT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DNTT
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1791
  • Target name
    DNTT
  • Target description
    DNA nucleotidylexotransferase
  • Target synonyms
    DNA nucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; TDT; terminal addition enzyme; terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; terminal transferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04053
  • Protein Name
    DNA nucleotidylexotransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TdT using anti-TdT antibody (PB9162). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TDT protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 39 kDa. The expected band size for TdT is at 39 kDa.
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