Anti-Do (b) [Mima-73]

Absolute Antibody

Anti-Do (b) [Mima-73]

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The specificity of this antibody has been confirmed in haemagglutination studies, where it reacts with Do(a-b+) and Do(a+b+) RBCs, but not with Do(a+b-) RBCs (Grodecka et al, 2012). The epitope recognised by this antibody is resistant to treatment with papain, alpha-chymotrypsin and neuraminidase, and sensitive to treatment with trypsin and DTT (Grodecka et al, 2012). This antibody has been used, as part of a panel of antibodies specific for different epitopes on the Dombrock glycoprotein, in flow cytometric and haemagglutination analysis of human red blood cells (Mayer et al, 2010; Costa et al, 2010).

Flow Cytometry

FC

Research Use Only

Monoclonal

Mima-73

ART4

ADP-ribosyltransferase 4 (inactive) (Dombrock blood group)

ADP-ribosyltransferase 4 (DO blood group); ADP-ribosyltransferase 4 (Dombrock blood group); ADP-ribosyltransferase C2 and C3 toxin-like 4; ARTC4; CD297; DO; Do glycoprotein; DO/ART4; DOK1; Dombrock blood group carrier molecule; Dombrock glycoprotein; ecto-ADP-ribosyltransferase 4; mono-ADP-ribosyltransferase 4; NAD(+)--protein-arginine ADP-ribosyltransferase 4; NAD(P)(+)--arginine ADP-ribosyltransferase 4; truncated Dombrock blood group protein

Human

IgG1

Ecto-ADP-ribosyltransferase 4

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2c format, for improved compatibility with existing reagents, assays and techniques.

Human

This antibody was raised by immunising BALB/c mice with human embryonic kidney 293T cells transfected to express high levels of Dombrock b.

-20°C,2°C to 8°C

12352203