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Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles.

Anti-DOCK1 Antibody

HPA053277
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetDOCK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DOCK1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1793
  • Target name
    DOCK1
  • Target description
    dedicator of cytokinesis 1
  • Target synonyms
    180 kDa protein downstream of CRK; ced5; dedicator of cytokinesis protein 1; DOCK180; DOwnstream of CrK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14185
  • Protein Name
    Dedicator of cytokinesis protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DOCK180/DOCK1 using anti-DOCK180/DOCK1 antibody (A03440-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK180/DOCK1 antigen affinity purified polyclonal antibody (Catalog # A03440-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DOCK180/DOCK1 at approximately 215 kDa. The expected band size for DOCK180/DOCK1 is at 215 kDa.
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