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Anti-DOCK4 Antibody

ER1802-17
HUABIO
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
DownloadDatasheet
Product group Antibodies
Price on request
100 ul
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Overview

  • Supplier
    HUABIO
  • Product Name
    Anti-DOCK4 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB,IF-Cell,IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/mL.
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DOCK4 using anti-DOCK4 antibody (A05916-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat L6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK4 antigen affinity purified polyclonal antibody (Catalog # A05916-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DOCK4 at approximately 250 kDa. The expected band size for DOCK4 is at 225 kDa.
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