Anti-DOTA (metal-bound) [2D12.5]

Absolute Antibody

Anti-DOTA (metal-bound) [2D12.5]

7

This antibody binds metal bound DOTA (Tetraxetan) and also shows binding affinity for Yttrium(III) labeled DOTA, (S) nitro benzyl-DOTA chelates and Janus-DOTA (a bivalent form of DOTA). DOTA is a chelating agent which can be labelled with radioactive yttr

The original mouse IgG1 antibody binds DOTA chelates of all the rare earths with approximately Kd ~ 10(-8) M, making it useful for the capture of probe molecules with a variety of properties (PMID: 15546206). The original IgG1 version of this antibody was used in the pretargeting for radioimmunotherapy in BALB/c mice with KHJJ tumors (PMID: 7954426). In vivo clearance studies have demonstrated that 74-96% of 2D12.5 bound to metal chelates is present 24 hours after administration of the bound complex (US7528235). An engineered version of this antibody with a single cysteine residue at position 54, 55 or 56 of the heavy chain, a site proximal to the proteins binding site, enables formation of weakly electrophilic metal complexes of (S)-2-(4-acrylamidobenzyl)-DOTA (AABD) that may bind and form permanent linkages (PMID: 15546206; 15546207). The heavy chain G54C mutant had a small set of electrophilic ligands, differences in reactivity indicated that the substituents near the electrophilic atom can be important determinants of permanent binding (PMID: 15546207).

Other Application

Radioimmunotherapy

Research Use Only

Monoclonal

2D12.5

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Other Species

Species independent

The original antibody was generated by immunizing BALB/c mice with KLH-21T-BAD-Y(III).

-20°C,2°C to 8°C

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