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Immunohistochemical staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.
Immunohistochemical staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.
Immunohistochemical staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.

Anti-DPYSL2 Antibody

HPA002381
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetDPYSL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DPYSL2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1808
  • Target name
    DPYSL2
  • Target description
    dihydropyrimidinase like 2
  • Target synonyms
    collapsin response mediator protein hCRMP-2; CRMP2; CRMP-2; DHPRP2; dihydropyrimidinase-related protein 2; DRP2; DRP-2; N2A3; ULIP2; ULIP-2; unc-33-like phosphoprotein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16555
  • Protein Name
    Dihydropyrimidinase-related protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded mouse brai; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CRMP2/TOAD64 Polyclonal Antibody (bs-1790R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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Figure 1. Western blot analysis of CRMP2/DPYSL2 using anti-CRMP2/DPYSL2 antibody (A02860-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRMP2/DPYSL2 antigen affinity purified polyclonal antibody (Catalog # A02860-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRMP2/DPYSL2 at approximately 65 kDa. The expected band size for CRMP2/DPYSL2 is at 65 kDa.
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