Anti-DR4 [mAY4]

Absolute Antibody

Anti-DR4 [mAY4]

9

mAY4 was shown to bind DR4 by ELISA (Sung et al, 2009). These experiments also showed that mAY4 did not react with other homologous death receptors, including DR5, decoy receptor (DcR) 1, DcR2, FAS receptor, and mTR (a mouse ortholog of human DR4 and DR5)

mAY4 was shown to bind DR4, but not other related homologs, using ELISAs and SPR (Sung et al, 2009). The mAb and scFv-reformatted version were also used in cell viability assays for assessment of tumouricidal activity. More recently, the mAY4 CDRs were humanised and expressed as an scFv-reformatted antibody fragment (Lee et al, 2010), although the humanised version had a 10-fold lower affinity for DR4.

Western Blot, ELISA, Other Application

Research Use Only

Monoclonal

mAY4

TNFRSF10A

TNF receptor superfamily member 10a

APO2; CD261; cytotoxic TRAIL receptor; death receptor 4; DR4; TNF-related apoptosis-inducing ligand receptor 1; TRAIL receptor 1; TRAILR1; TRAIL-R1; TRAILR-1; tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a

Human

IgG1

Tumor necrosis factor receptor superfamily member 10A

This full-length, chimeric human antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203